osmosis

Description

Osmosis is defined as the movement of water across a selectively permeable membrane from an area of high concentration to an area of lowconcentration. In order to demonstrate this process, students used dialysis tubing and solutions with varying concentrations.  Six student groupsmade three separate dialysis sacs containing a 15% glucose solution. Each bag’s initial volume was recorded (Table 1). Afterwards, each dialysis sac was placed in one of three separate beakers containing a different solution.Beaker A contained distilled water. Beaker B contained a 15% glucose solution. Beaker C contained a 30% glucose solution. After 45 minutes, the bags were removed from the solution, dried off and the final volume was measured. The final volume was recorded in Table 1.

Condition 1

Condition 2

Condition 3

Beaker A

Beaker B

Beaker C

Group

Initial

Volume

(mL)

Final

Volume

(mL)

Volume

Change

(mL)

Group

Initial

Volume

(mL)

Final

Volume

(mL)

Volume

Change

(mL)

Group

Initial

Volume

(mL)

Final

Volume

(mL)

Volume

Change

(mL)

1

8.1

10.6

1

7.5

7.5

1

7.2

6.1

2

6.4

8.2

2

7.7

7.7

2

7.5

6.4

3

7.2

9.4

3

6.5

6.5

3

8.1

6.2

4

7.5

9.9

4

8.8

8.8

4

6.2

4.5

5

6.9

8.8

5

7.1

7.1

5

7.6

5.2

6

6.4

8.4

6

6.4

6.4

6

6.4

4.5

TOTAL:

TOTAL:

TOTAL:

MEAN:

MEAN:

MEAN:

Table 1

1. State the null hypothesis:

 

 

2. State three alternative hypotheses based on the tonicity for each condition.

 

 

3. What is the independent variable?

 

 

4. What is the dependent variable?

 

5. In Table 1, total the volume changes seen by the groups under each condition. Next, calculate the mean (average) of each volume change.  The mean is calculated by calculating the total and dividing by the total number of data points. Record your results in Table 1.

6. Make two column graphs to illustrate your data. Be sure to label the axes of the graph and use the appropriate units, if necessary.

A) Make a column graph illustrating the initial and final volume of each condition. Insert chart below.

B) Make a column graph demonstrating the mean volume change of each condition. Insert chart below.

7. Based on the data and your knowledge of osmosis and tonicity, scientifically explain what occurred under each condition.

 

8. Does the data support or reject your null hypothesis?  Explain.

 

 

9. Does the data support your alternative hypotheses? Explain.

 

 

 

 

10. To further demonstrate diffusion, students made a dialysis sac containing a 30% glucose solution and placed it in Beaker A. Do you expect that the mean final volume of the sac containing a 30% glucose solution will be higher, lower, or the same as the mean final volume of the sac containing a 15% glucose solution? Explain.
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