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BIO 120 EHS Benedict Test to Detect Presence Reduction of Sugar Lab Report

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Question Description

  1. State and interpret your results for the enzyme experiment at each of the four temperatures.
    • Did your results make sense? Why or why not?
    • What did your results tell you about enzyme activity under each temperature condition?
    • Why did invertase behave the way it did in each condition?
    • What conclusions can you draw about the activity of invertase in biological organisms?
    • Use your textbook and other resources to better understand and explain your results.
    • ANY INFORMATION YOU DERIVE FROM ANOTHER SOURCE MUST BE CITED.
  2. State and interpret your results for the enzyme experiment at each of the four pH levels.
    • Did your results make sense? Why or why not?
    • What did your results tell you about enzyme activity under each condition?
    • Why did invertase behave the way it did in each condition?
    • What conclusions can you draw about the activity of invertase in biological organisms?
    • Use your textbook and other resources to better understand and explain your results.
    • ANY INFORMATION YOU DERIVE FROM ANOTHER SOURCE MUST BE CITED.
  3. Create a diagram or flow chart showing the experimental procedure for the enzyme temperature and pH experiments.
  4. Explain the chemistry behind Benedict’s reagent. How is Benedict’s reagent affected by the presence of reducing sugars? Chemically speaking, why does this change take place?
  5. Given your answers to question 4, why do we use Benedict’s reagent to test for the activity of invertase?
  6. Draw the chemical equation for the breakdown of sucrose.
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